The regulatory role of DNA supercoiling in nucleoprotein complex assembly and genetic activity

被引：20
|
作者：
Muskhelishvili G. [1 ]
Travers A. [2 ,3 ]
机构：
[1] Jacobs University Bremen, Bremen
[2] MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge
[3] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge
来源：
Biophysical Reviews | 2016年 / 8卷 / Suppl 1期
基金：
英国医学研究理事会;
关键词：
Chromatin organization; DNA supercoiling; DNA translocases; DNA untwisting; DNA writhing; Superhelicity gradients;
D O I：
10.1007/s12551-016-0237-3
中图分类号：
学科分类号：
摘要：
We argue that dynamic changes in DNA supercoiling in vivo determine both how DNA is packaged and how it is accessed for transcription and for other manipulations such as recombination. In both bacteria and eukaryotes, the principal generators of DNA superhelicity are DNA translocases, supplemented in bacteria by DNA gyrase. By generating gradients of superhelicity upstream and downstream of their site of activity, translocases enable the differential binding of proteins which preferentially interact with respectively more untwisted or more writhed DNA. Such preferences enable, in principle, the sequential binding of different classes of protein and so constitute an essential driver of chromatin organization. © 2016, International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag Berlin Heidelberg.
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页码：5 / 22
页数：17
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