Toxin-binding properties of cytochrome P450 in Saccharomyces cerevisiae and Kluyveromyces marxianus

被引：0
|
作者：
Kathryn H. Engler
Steven L. Kelly
Raymond D. Coker
Ivor H. Evans
机构：
[1] University of Greenwich,Institute of Biological Sciences
[2] University of Wales Aberystwyth,Natural Resources Institute
[3] Chatham Maritime,undefined
来源：
Biotechnology Letters | 2000年 / 22卷
关键词：
bioassay; cytochrome P450; mycotoxins;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Microsomes from Kluyveromyces marxianus GK1005 examined by carbon monoxide difference spectroscopy showed no evidence of cytochrome P450, in contrast to microsomes isolated from a control strain of Saccharomyces cerevisiae. Benzo[a]pyrene produced a typical Type I-binding spectrum with microsomes of both yeasts, with Ks values of 82 μM (S. cerevisiae) and 70 μM (K. marxianus). While aflatoxin B1 generated a typical Type I-binding spectrum with microsomes from S. cerevisiae (Ks of 178 μM), the toxin did not produce a recognisable binding spectrum with microsomes from K. marxianus.
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页码：3 / 8
页数：5
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