Visualizing SNVs to quantify allele-specific expression in single cells

被引：1
|
作者：
Levesque M.J. [1 ]
Ginart P. [1 ,2 ]
Wei Y. [1 ]
Raj A. [1 ]
机构：
[1] Department of Bioengineering, University of Pennsylvania, Philadelphia, PA
[2] Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA
来源：
Nature Methods | 2013年 / 10卷 / 9期
关键词：
D O I：
10.1038/nmeth.2589
中图分类号：
学科分类号：
摘要：
We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons and introns on individual RNA transcripts with high efficiency. We used this method to quantify allelic expression in cell populations and in single cells, and also to distinguish maternal from paternal chromosomes in single cells. © 2013 Nature America, Inc. All rights reserved.
引用
收藏
页码：865 / 867
页数：2
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