Identification and analysis of expressed sequence tags present in xylem tissues of kelampayan (Neolamarckia cadamba (Roxb.) Bosser)

被引：0
|
作者：
Wei-Seng Ho
Shek-Ling Pang
Julaihi Abdullah
机构：
[1] Universiti Malaysia Sarawak,Forest Genomics and Informatics Laboratory, Department of Molecular Biology, Faculty of Resource Science and Technology
[2] Sarawak Forestry Corporation,Applied Forest Science and Industry Development Unit
来源：
Physiology and Molecular Biology of Plants | 2014年 / 20卷
关键词：
cDNA library; Expressed sequence tags (EST); Developing xylem; Wood formation; Lignin biosynthesis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The large-scale genomic resource for kelampayan was generated from a developing xylem cDNA library. A total of 6,622 high quality expressed sequence tags (ESTs) were generated through high-throughput 5’ EST sequencing of cDNA clones. The ESTs were analyzed and assembled to generate 4,728 xylogenesis unigenes distributed in 2,100 contigs and 2,628 singletons. About 59.3 % of the ESTs were assigned with putative identifications whereas 40.7 % of the sequences showed no significant similarity to any sequences in GenBank. Interestingly, most genes involved in lignin biosynthesis and several other cell wall biosynthesis genes were identified in the kelampayan EST database. The identified genes in this study will be candidates for functional genomics and association genetic studies in kelampayan aiming at the production of high value forests.
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页码：393 / 397
页数：4
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