Effects of UV-C radiation on Chlorella vulgaris, a biofilm-forming alga

Photosynthetic biofilms proliferating on heritage monuments represent a major threat for curators leading to biodegradation and esthetic issues. Previous studies demonstrated that UV-C, used as a tool for biofilm eradication, is a promising avenue to combat microbial proliferation. In this study, this environmentally friendly method was tested on biofilm-forming Chlorella vulgaris suspension. Algal physiological response to UV-C was then assessed. Results showed that > 10 kJ m−2 UV-C exposure was enough to directly kill cells whereas low UV-C exposure reduced quantum yield of photosystem II and inhibited both respiration and photosynthesis. Clear relationships between UV-C exposure times and physiological responses were found. In addition, the use of VIS-light after UV-C treatment enhances chlorophyll bleaching. Our findings contribute to a better understanding of the physiological responses of Chlorella vulgaris to UV-C radiation allowing thus an optimization of the UV-C treatment reported in our previous studies.