DNA separation with low-viscosity sieving matrix on microfabricated polycarbonate microfluidic chips

被引：0
|
作者：
Mei-Ying Ye
Xue-Feng Yin
Zhao-Lun Fang
机构：
[1] Zhejiang University,Institute of Microanalytical Systems, Department of Chemistry
来源：
Analytical and Bioanalytical Chemistry | 2005年 / 381卷
关键词：
Hot embossing; Polycarbonate; Microchip; DNA; Electrophoresis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Microfluidic devices have been fabricated on polycarbonate (PC) substrates by use of a hot embossing method using a silicon master template. By adding auxiliary lines around the functional channel on the silicon master, its lifetime was significantly prolonged and the bonding strength of the PC cover plate to the microfluidic chip was greatly improved. More than 300 polycarbonate microfluidic chips have been replicated with the same silicon mold. CE separation of Φ X-174/HaeIII DNA restriction fragments, with high resolution efficiency and good reproducibility, was achieved on these devices using the low-viscosity sieving matrix HPMC-50. Temperature was found to have a significant effect on separation efficiency.
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收藏
页码：820 / 827
页数：7
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