Development of an electrochemical DNA biosensor for detection of specific Mycobacterium tuberculosis sequence based on poly(L-glutamic acid) modified electrode

被引：0
|
作者：
MERVE YESIL
SONER DONMEZ
FATMA ARSLAN
机构：
[1] Gazi University,Department of Chemistry, Faculty of Science
[2] Mehmet Akif Ersoy University,Bucak School of Health
来源：
Journal of Chemical Sciences | 2016年 / 128卷
关键词：
Poly(L-glutamic acid); DNA sensor; Mycobacteriumtuberculosis.;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
An electrochemical DNA biosensor was developed by avidin-biotin interaction of a biotinylated probe and avidin-attached, poly(L-glutamic) acid coated pencil graphite electrode (PGA/PGE) for detection of specific Mycobacterium tuberculosis DNA sequence. The discrimination of fully complementary hybridization and mismatch hybridization was carried out by electrochemical reduction current of Meldola’s Blue (MDB) in square wave voltammetry (SWV). The calibration graph of the DNA biosensor was linear between 1.5–12.5 nM and the detection limit was calculated as 1.3 nM. The proposed biosensor successfully discriminated short and long oligonucleotides related to DNA sequence of Mycobacterium tuberculosis in optimal condition.
引用
收藏
页码：1823 / 1829
页数：6
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