Preliminary experience in external quality control of RT-PCR PML-RARα detection in promyelocytic leukemia

被引：0

作者：

P Bolufer

E Barragán

MA Sánz

G Martín

R Bornstein

D Colomer

MD Delgado

M González

I Marugan

J Román

MT Gómez

E Anguita

D Diverio

Ch Chomienne

M Briz

机构：

[1] Laboratorio de Biología Molecular,

[2] Biopatología Clínica,undefined

[3] Unidad de Oncohematología,undefined

[4] Servicio de Hematología,undefined

[5] Hospital Universitario La Fe,undefined

[6] Unidad de Criobiología y Biología Molecular,undefined

[7] Servicio de Hematología,undefined

[8] Hospital 12 de Octubre,undefined

[9] Sección de Hematología,undefined

[10] Area Biología Molecular,undefined

[11] H Clínico De Barcelona,undefined

[12] Biología Molecular,undefined

[13] Hematología,undefined

[14] Hospital Marques de Valdecilla,undefined

[15] Laboratorio de Inmunopatología y Biología Molecular,undefined

[16] Hematología,undefined

[17] H Clínico Universitario,undefined

[18] Laboratorio de Hematología,undefined

[19] Servicio de Hematología y Oncología Médica,undefined

[20] Hospital Clínico Universitario,undefined

[21] Unidad de Biología Molecular,undefined

[22] Hematología,undefined

[23] Hospital Reina Sofía,undefined

[24] Biología Molecular,undefined

[25] Hematología,undefined

[26] H Nuestra Señora del Pino,undefined

[27] Hematología,undefined

[28] Hematología y Hemoterapia,undefined

[29] H Universitario San Carlos,undefined

[30] Laboratorio di Diagnostica Moleculares Oncoematologica,undefined

[31] Sezione Ematologia/Biologia Umana,undefined

[32] Universitá Degli Studi ‘La Sapienza’,undefined

[33] Laboratoire de Biologie Cellulaire Hematopoietique,undefined

[34] Institut d’Hématologie,undefined

[35] Hôpital Saint-Louis,undefined

[36] Biologia Molecular,undefined

[37] Hematologia,undefined

[38] Puerta de Hierro,undefined

来源：

Leukemia | 1998年 / 12卷

关键词：

PML/RARαrearrangement; RT-PCR; external quality assessment; acute promyelocytic leukemia;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

To standardize the results obtained in PML/RARα RT-PCR detection by laboratories of hospitals involved in the Spanish Program for Treatment of Hematological Malignancies (PETHEMA) LPA-96, designed for the treatment of acute promyelocytic leukemia (APL), cDNA samples obtained by reverse transcription of RNA from bone marrow samples of patients with APL were sent to participating laboratories. During the first year of this external quality assessment trial nine samples were tested by a maximum of 12 laboratories. The control gene was satisfactorily amplified in 90% of the samples (62 of 69 samples), supporting the adequacy of the cDNA to be used as control sample. There was an 83% concordance between laboratories for PML/RARα detection with similar results for the type of PML/RRα rearrangements. However, 17% disagreement still remained, attributable to low sensitivity or inadequacy of methods followed. The results stressed the need for implementation of an external quality assessment scheme to ensure the standardization of the results.

引用

页码：2024 / 2028

页数：4

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