Isolation and Properties of Cellobiase from Penicillium verruculosum

被引：0

作者：

I. N. Zorov

A. V. Gusakov

V. A. Baraznenok

A. O. Bekkarevich

O. N. Okunev

A. P. Sinitsyn

E. G. Kondrat'eva

机构：

[1] Moscow State University,Faculty of Chemistry

[2] Institute of Biochemistry and Physiology of Microorganisms,undefined

[3] RAS,undefined

来源：

Applied Biochemistry and Microbiology | 2001年 / 37卷

关键词：

Sugar; Molecular Weight; Hydrolysis; Kinetic Parameter; Enzymatic Hydrolysis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Cellobiase (β-D-glucosidase) with a molecular weight of 100 kDa and pI 5.2 was isolated from the cellulolytic system of Penicillium verruculosum. Kinetic parameters of enzymatic hydrolysis of cellobiose, gentiobiose, sophorose, and synthetic substrates, i. e. methylumbelliferyl and p-nitrophenyl sugar derivatives were determined. Glucose and D-glucose-δ-lactone competitively inhibited cellobiase (Ki0.19 mM and 17 μM, respectively). Glucosyl transfer reactions were studied with cellobiose as a single substrate and in the mixture of cellobiose and methylumbelliferyl cellobioside. The product composition was determined in these systems. The ratio of hydrolysis and transfer reaction rates for cellobiose conversion was calculated.

引用

页码：587 / 593

页数：6

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