A Simple Method for Identifying Plant/T-DNA Junction Sequences Resulting from Agrobacterium-mediated DNA Transformation

被引：0
|
作者：
Yuanxiang Zhou
Ronald J. Newton
Jean H. Gould
机构：
[1] Texas A&M University,Department of Forest Science
来源：
Plant Molecular Biology Reporter | 1997年 / 15卷
关键词：
Junction sequence; transformation; Agrobacterium tumefaciens;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Genomic integration of transferred T-DNA is traditionally analyzed by Southern hybridization; however, these analyses often do not provide sufficient information pertaining to the transformation event. Analysis of the junction sequences spanning the region between the T-DNA borders and plant genomic DNA, give a clear demonstration of genomic integration. The procedures available for border junction analysis can be problematic, therefore a simplified method was developed for plants transformed by Agrobacterium tumefaciens harboring the binary vector with pBI121 backbone.
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页码：246 / 254
页数：8
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