Mono-2, 3 or 6-Hydroxy Methylated β-Cyclodextrin (Eicosa-O-methyl-β-cyclodextrin) Isomers as Chiral Stationary Phases for Capillary GC

This work deals with the evaluation of three monohydroxy methylated β-cyclodextrin isomers as chiral selectors for capillary gas chromatography. Chromatographic properties of these stationary phases have been investigated in terms of efficiency, inertness and polarity. Injections of homologous series of hydantoin derivatives and 1-phenylethan-1-ol have highlighted the predominant influence of hydroxyl groups in 2 or 3 positions on chiral discrimination. By contrast, presence of hydroxyl groups in 6-O-position did not significantly influence the enantioseparation ability. Moreover, Tiso calculated on TRIMEB-2-OH in OV1701-OH and on pure TRIMEB-2-OH for 5-methyl-5-alkyl hydantoin derivatives were notably different indicating that polysiloxan–selector interactions could not be neglected.