Rapid estimation and quantification of sucrose content in fruit juices using Fourier transform infrared–attenuated total reflectance (FTIR–ATR) spectroscopy

An uncomplicated and rapid procedure has been developed for the quantitative analysis of sucrose in fruit samples (grape, pineapple, mango) through attenuated total reflectance–Fourier transform infrared absorbance measurements (ATR–FTIR). FTIR analysis takes considerably reduced time compared to the other classical methods. To calibrate the method, we used firstly, different concentrations of pure sucrose (from 1 to 5 %) and registered their IR maximal wavenumbers and peak intensity. The spectral peak of sucrose for each sample lies between 1057 and 1061 cm−1. DNS method was used to analyse the content of sucrose by using spectrophotometry. The wave length used for analysing is 540 nm. Also high performance liquid chromatography was used to analyse the sucrose content in the fruit juices. By comparing the retention time of sucrose standards and the sample juices, sucrose concentration was identified and quantified. The results of all three experiments/techniques support each other by justifying that the mango has the high content of sucrose followed by pineapple and grape.