The effects of jasmonic acid and methyl jasmonate on rosmarinic acid production in Mentha × piperita cell suspension cultures

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作者
Justyna Krzyzanowska
Anna Czubacka
Lukasz Pecio
Marcin Przybys
Teresa Doroszewska
Anna Stochmal
Wieslaw Oleszek
机构
[1] State Research Institute,Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation
[2] State Research Institute,Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation
关键词
 × ; Cell suspension cultures; Jasmonic acid; Methyl jasmonate; Elicitation; Rosmarinic acid;
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摘要
The effects of two elicitors: jasmonic acid and methyl jasmonate on cell growth as well as on rosmarinic acid accumulation in cell suspension cultures of Mentha × piperita were investigated. The highest rosmarinic acid accumulation 117.95 mg g−1 DW (12% DW) was measured 24 h after addition of 100 μM methyl jasmonate. A similar concentration 110.12 mg g−1 DW was detected 48 h after application of 200 μM jasmonic acid. Those values were nearly 1.5 times higher compared to the control sample, without elicitation. There was no substantial influence of elicitors on rosmarinic acid secretion into the culture media. Extracellular concentrations of rosmarinic acid were similar to the values from the control variants. It was documented that suspension cultures of M. piperita treated with elicitors showed a decrease in biomass accumulation when compared to the control.
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页码:73 / 81
页数:8
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