Faster, sharper, and deeper: structured illumination microscopy for biological imaging

被引:0
|
作者
Yicong Wu
Hari Shroff
机构
[1] National Institutes of Health,Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering
来源
Nature Methods | 2018年 / 15卷
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摘要
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.
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页码:1011 / 1019
页数:8
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