Faster, sharper, and deeper: structured illumination microscopy for biological imaging
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作者:
Yicong Wu
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机构:National Institutes of Health,Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering
Yicong Wu
Hari Shroff
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机构:National Institutes of Health,Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering
Hari Shroff
机构:
[1] National Institutes of Health,Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering
来源:
Nature Methods
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2018年
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15卷
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摘要:
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.
机构:
Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32304 USA
Florida State Univ, Dept Biol Sci, Tallahassee, FL 32304 USAFlorida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32304 USA
Allen, John R.
Ross, Stephen T.
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机构:
Nikon Instruments Inc, Melville, NY 11747 USAFlorida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32304 USA
Ross, Stephen T.
Davidson, Michael W.
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机构:
Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32304 USA
Florida State Univ, Dept Biol Sci, Tallahassee, FL 32304 USAFlorida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32304 USA