Evaluation of Glycosyl Hydrolases in the Secretome of Aspergillus fumigatus and Saccharification of Alkali-Treated Rice Straw

被引：0
|
作者：
Manju Sharma
Rohit Soni
Asiya Nazir
Harinder Singh Oberoi
Bhupinder Singh Chadha
机构：
[1] Guru Nanak Dev University,Department of Microbiology
[2] Central Institute of Post Harvesting Technology,undefined
来源：
Applied Biochemistry and Biotechnology | 2011年 / 163卷
关键词：
Secretome; Peptide mass fingerprinting; Glycosyl hydrolases; Activity detection of CBHI/EGI and β-glucosidases in 2DE gels; Saccharification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A thermotolerant Aspergillus fumigatus strain isolated from composting pile of mixed industrial waste was found to produce a spectrum of cellulase and hemicellulases when cultured on rice straw solidified substrate. The two-dimensional electrophoresis (2DE) resolved the secretome into 57 distinct protein spots. The zymograms developed against 2DE gels identified the presence of three β-glucosidases and five CBHI/EGI isoforms in the secretome. The peptide mass fingerprinting of 17 protein spots by liquid chromatography mass spectrometry characterized the secretome into different glycosyl hydrolase families. The enzyme cocktail produced by A. fumigatus was capable of efficient hydrolysis of alkali pretreated rice straw (at 7% and 10% w/v) resulting in 95% and 91% saccharification, respectively.
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页码：577 / 591
页数：14
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