Improved paclitaxel production in a two-phase suspension culture of Taxus cuspidata using silicone cubes

被引：0

作者：

Kim C.-H. ^{[1
]}

Hong S.-H. ^{[1
]}

Son S.-H. ^{[2
]}

Chung I.-S. ^{[1
]}

机构：

[1] Department of Genetic Engineering, Kyung Hee University

[2] Biotechnology Division, Forestry Research Institute

来源：

Biotechnology and Bioprocess Engineering | 1999年 / 4卷 / 4期

关键词：

Paclitaxel; Silicone cube; Suspension culture; Taxus cuspidata; Two-phase culture;

D O I：

10.1007/BF02933752

中图分类号：

学科分类号：

摘要：

Two-phase cultures of Taxus cuspidata were performed using silicone cubes as a second phase in shake flasks for paclitaxel production. Among various taxanes, paclitaxel was selectively adsorbed on the silicon cubes. When silicone cubes were added to suspension culture of Taxus cuspidata, paclitaxel production increased about 45 folds. The maximum paclitaxel production was 3.95 mg/L when 10% of silicone cubes were added to the culture at the 7th day from inoculation.

引用

页码：273 / 276

页数：3

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