Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli

被引：0
|
作者：
Misun Won
Soyoun Kim
Seunghwan Lee
Chuljung Kim
Hyunsu Kim
Moohyung Jun
Kyung Bin Song
机构：
[1] Korea Research Institute of Bioscience and Biotechnology,Cell Cycle and Signal Research Unit
[2] Departments of Food Science and Technology,Veterinary Medicine
[3] Chungnam National University,undefined
来源：
Biotechnology Letters | 2000年 / 22卷
关键词：
MBP-fusion protein; SEF 17;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied.
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页码：1165 / 1168
页数：3
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