Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E-coli

被引：1
|
作者：
Won, M
Kim, S
Lee, S
Kim, C
Kim, H
Jun, M
Song, KB [1 ]
机构：
[1] Chungnam Natl Univ, Dept Food Sci & Technol, Taejon 305764, South Korea
[2] Chungnam Natl Univ, Dept Vet Med, Taejon 305764, South Korea
[3] Korea Res Inst Biosci & Biotechnol, Cell Cycle & Signal Res Unit, Taejon, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 14期
关键词：
MBP-fusion protein; Salmonella entiritidis; SEF; 17;
D O I：
10.1023/A:1005605610004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied.
引用
收藏
页码：1165 / 1167
页数：3
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