High-efficiency transformation of Lycium barbarum mediated by Agrobacterium tumefaciens and transgenic plant regeneration via somatic embryogenesis

被引：0
|
作者：
Z. Hu
J. Yang
G. Guo
G. Zheng
机构：
[1] Institute of Cell Biology,
[2] Lanzhou University,undefined
[3] Lanzhou 730000,undefined
[4] PR China,undefined
[5] Present address: Department of Biology,undefined
[6] Shantou University,undefined
[7] Shantou 515063,undefined
[8] Guangdong Province,undefined
[9] PR China,undefined
[10] Present address: College of Bioengineering,undefined
[11] Chongqing University,undefined
[12] Chongqing 400044,undefined
[13] PR China,undefined
来源：
Plant Cell Reports | 2002年 / 21卷
关键词：
Lycium barbarum Agrobacterium tumefaciens Transgenic plants Somatic embryogenesis;
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学科分类号：
摘要：
We have developed a reliable and high-frequency system of transformation and regeneration via somatic embryogenesis (SE) of Lycium barbarum. Leaf segments were co-cultivated with Agrobacterium tumefaciens EHA101 (pIG121Hm) carrying the neomycin phosphotransferase II gene as a selectable marker and an intron-β-glucuronidase (GUS) gene as a reporter marker. On the medium for callus-induction, which contained 50 mg l–1 kanamycin (Km), approximately 60% of the explants produced kanamycin-resistant (KmR) calli. After three subcultures on the same selective medium, KmR calli were transferred onto SE induction medium containing 25 mg l–1 Km, where they produced numerous somatic embryos (166 g–1fresh weight callus) that subsequently developed into KmR plantlets. Compared with the untransformed control calli, the transformed calli maintained their higher frequency of SE up to 16 months after transformation. GUS staining and polymerase chain reaction and Southern blot analyses confirmed the integration of the T-DNA into the plant genome.
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页码：233 / 237
页数：4
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