A high-quality catalog of the Drosophila melanogaster proteome

被引:0
|
作者
Erich Brunner
Christian H Ahrens
Sonali Mohanty
Hansruedi Baetschmann
Sandra Loevenich
Frank Potthast
Eric W Deutsch
Christian Panse
Ulrik de Lichtenberg
Oliver Rinner
Hookeun Lee
Patrick G A Pedrioli
Johan Malmstrom
Katja Koehler
Sabine Schrimpf
Jeroen Krijgsveld
Floyd Kregenow
Albert J R Heck
Ernst Hafen
Ralph Schlapbach
Ruedi Aebersold
机构
[1] Center for Model Organism Proteomes,Department of Biomolecular Mass Spectrometry
[2] University of Zurich,undefined
[3] Winterthurerstrasse 190,undefined
[4] Functional Genomics Center,undefined
[5] ETH and University of Zurich,undefined
[6] Winterthurerstrasse 190,undefined
[7] Y55L70,undefined
[8] Institute for Molecular Systems Biology,undefined
[9] Swiss Federal Institute of Technology,undefined
[10] ETH Honggerberg,undefined
[11] Institute for Systems Biology,undefined
[12] Center for Biological Sequence Analysis,undefined
[13] BioCentrum-DTU,undefined
[14] Technical,undefined
[15] University of Denmark,undefined
[16] Kemitorvet,undefined
[17] Building 208,undefined
[18] LEO Pharma,undefined
[19] Industriparken 55,undefined
[20] Institute for Molecular Biology,undefined
[21] University of Zurich,undefined
[22] Winterthurerstrasse 190,undefined
[23] Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences,undefined
[24] Utrecht University,undefined
[25] Sorbonnelaan 16,undefined
[26] Faculty of Science,undefined
[27] University of Zurich,undefined
[28] Winterthurerstrasse 190,undefined
来源
Nature Biotechnology | 2007年 / 25卷
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摘要
Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanogaster proteome by detecting 9,124 proteins from 498,000 redundant and 72,281 distinct peptide identifications. This unprecedented high proteome coverage for a complex eukaryote was achieved by combining sample diversity, multidimensional biochemical fractionation and analysis-driven experimentation feedback loops, whereby data collection is guided by statistical analysis of prior data. We show that high-quality proteomics data provide crucial information to amend genome annotation and to confirm many predicted gene models. We also present experimentally identified proteotypic peptides matching ∼50% of D. melanogaster gene models. This library of proteotypic peptides should enable fast, targeted and quantitative proteomic studies to elucidate the systems biology of this model organism.
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页码:576 / 583
页数:7
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