A model of BmK CT in inhibiting glioma cell migration via matrix metalloproteinase-2 from experimental and molecular dynamics simulation study

被引：0

作者：

Yue-Jun Fu

Na An

Kok-Gan Chan

Yan-Bo Wu

Shu-Hua Zheng

Ai-Hua Liang

机构：

[1] Shanxi University,Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology

[2] University of Malaya,Institute of Biological Sciences, Division of Genetics and Molecular Biology, Faculty of Science

[3] Shanxi University,Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science

来源：

Biotechnology Letters | 2011年 / 33卷

关键词：

BmK CT; Glioma cells; Matrix metalloproteinase-2; Docking simulation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The significance of BmK CT, a key chlorotoxin-like peptide isolated from the scorpion venom of Buthus martensii Karsch, is a novel blocker of the chloride ion channel and matrix metalloproteinase-2 (MMP-2). Site-directed mutagenesis of BmK CT, wound healing assay, gelatin zymography assay and computational simulation highlight the importance of electrostatic contribution to BmK CT–MMP-2 catalytic domain complex and a model of BmK CT–MMP-2 catalytic domain complex is therefore proposed. This is the first documentation of the structural mechanism of in the inhibition of glioma cell migration by BmK CT and may lead to the molecular design of specific inhibitors of MMP-2.

引用

页码：1309 / 1317

页数：8