Temporal and subcellular localization of infectious pancreatic necrosis virus structural proteins

被引：0
|
作者：
J. C. Espinoza
A. Hjalmarsson
E. Everitt
J. Kuznar
机构：
[1]  Laboratorio de Bioquímica y Virología,
[2] Instituto de Ciencias Biológicas y Químicas,undefined
[3] Facultad de Ciencias,undefined
[4] Universidad de Valparaíso,undefined
[5] Chile,undefined
[6]  Department of Microbiology,undefined
[7] Lund University,undefined
[8] Sweden,undefined
来源：
Archives of Virology | 2000年 / 145卷
关键词：
Monoclonal Antibody; Cytosol; Subcellular Localization; Inclusion Body; Tubular Structure;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
 The temporal subcellular localization of the structural proteins VP2 and VP3 of infectious pancreatic necrosis virus was analyzed with monoclonal antibodies conjugated with fluorochromes. Early in the infection both proteins were colocalized in the cytosol, at later times, VP2 was visualized as inclusion bodies around the nuclei of the cells and, sometimes, it was found in elongated tubular structures that might correspond to the type I tubules seen in cells infected with another Birnavirus. As VP2 is a glycosylated protein we determined its subcellular localization compared with that of ER and Golgi probes. These results suggest that VP2 is glycosylated freely in the cytoplasm.
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页码：739 / 748
页数：9
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