The origin of genomic N6-methyl-deoxyadenosine in mammalian cells

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作者：
Michael U. Musheev
Anne Baumgärtner
Laura Krebs
Christof Niehrs
机构：
[1] Institute of Molecular Biology gGmbH,
[2] Division of Molecular Embryology,undefined
[3] DKFZ-ZMBH Alliance,undefined
来源：
Nature Chemical Biology | 2020年 / 16卷
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摘要：
The proposal that N6-methyl-deoxyadenosine (m6dA) acts as an epigenetic mark in mammals remains controversial. Using isotopic labeling coupled to ultrasensitive mass spectrometry, we confirm the presence of low-level m6dA in mammalian DNA. However, the bulk of genomic m6dA originates from ribo-N6-methyladenosine, which is processed via the nucleotide-salvage pathway and misincorporated by DNA polymerases. Our results argue against m6dA acting as a heritable, epigenetic DNA mark in mammalian cells.
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页码：630 / 634
页数：4
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