Development and characterization of 38 novel EST-SSRs for the sea cucumber Apostichopus japonicus

被引：0
|
作者：
Wei Peng
Zhenmin Bao
Huixia Du
Ying Dong
Yameng Lv
Zunchun Zhou
Jingjie Hu
机构：
[1] Ocean University of China,Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences
[2] Liaoning Ocean and Fisheries Science Research Institute,Liaoning Key Lab of Marine Fishery Molecular Biology
来源：
Conservation Genetics Resources | 2009年 / 1卷
关键词：
EST; Microsatellite; Sea cucumber;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A total of 5728 sequences from the expressed sequence tag database of sea cucumber Apostichopus japonicus were mined for microsatellite sequences. A total of 151 ESTs containing microsatellites were identified. Of 93 primer pairs designed, 56 amplified expected products and 38 showed polymorphism in 48 individuals tested. The number of alleles ranged from 2 to 10, and the observed and expected heterozygosities varied from 0.000 to 0.900 and from 0.332 to 0.884, respectively. No significant linkage disequilibrium (LD) between pairs of loci was found and 21 loci significantly deviated from the Hardy–Weinberg equilibrium (HWE).
引用
收藏
相关论文
共 50 条
  • [41] Characterization and Expression Analysis of MicroRNAs in the Tube Foot of Sea Cucumber Apostichopus japonicus
    Wang, Hongdi
    Liu, Shikai
    Cui, Jun
    Li, Chengze
    Qiu, Xuemei
    Chang, Yaqing
    Liu, Zhanjiang
    Wang, Xiuli
    PLOS ONE, 2014, 9 (11):
  • [42] Physiological characterization of aerobic culturable bacteria in the intestine of the sea cucumber Apostichopus japonicus
    Zhang, Xiaochi
    Nakahara, Tomomi
    Murase, Shinji
    Nakata, Hideaki
    Inoue, Tetsushi
    Kudo, Toshiaki
    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2013, 59 (01): : 1 - 10
  • [43] Characterization of a metalloprotease involved in Vibrio splendidus infection in the sea cucumber, Apostichopus japonicus
    Zhang, Chi
    Liang, Weikang
    Zhang, Weiwei
    Li, Chenghua
    MICROBIAL PATHOGENESIS, 2016, 101 : 96 - 103
  • [44] Characterization of thirteen single nucleotide polymorphism markers in the sea cucumber (Apostichopus japonicus)
    Wen-Jing Sun
    Qi Li
    Ling-Feng Kong
    Conservation Genetics Resources, 2010, 2 : 141 - 144
  • [45] Characterization and expression analysis of a complement component gene in sea cucumber (Apostichopus japonicus)
    Zhong Chen
    Zunchun Zhou
    Aifu Yang
    Ying Dong
    Xiaoyan Guan
    Bei Jiang
    Bai Wang
    Journal of Ocean University of China, 2015, 14 : 1096 - 1104
  • [46] The development of 204 novel EST-SSRs and their use for genetic diversity analyses in cultivated alfalfa
    Zhou, Qiang
    Chen, Tianlong
    Wang, Yanrong
    Liu, Zhipeng
    BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2014, 57 : 227 - 230
  • [47] Collagen fibrils of sea cucumber (Apostichopus japonicus) are heterotypic
    Tian, Mo
    Xue, Changhu
    Chang, Yaoguang
    Shen, Jingjing
    Zhang, Yuying
    Li, Zhaojie
    Wang, Yanchao
    FOOD CHEMISTRY, 2020, 316
  • [48] Cryopreservation of sea cucumber Apostichopus japonicus (Selenka) sperm
    Shao, Ming Yu
    Zhang, Zhi Feng
    Yu, Li
    Hu, Jing Jie
    Kang, Kyoung Ho
    AQUACULTURE RESEARCH, 2006, 37 (14) : 1450 - 1457
  • [49] Coccidian Parasite in Sea Cucumber (Apostichopus japonicus) Ovaries
    Unuma, Tatsuya
    Tsuda, Noriko
    Sakai, Yuichi
    Kamaishi, Takashi
    Sawaguchi, Sayumi
    Itoh, Naoki
    Yamano, Keisuke
    BIOLOGICAL BULLETIN, 2020, 238 (01): : 64 - 71
  • [50] Development of EST-SSRs in the Mediterranean blue mussel, Mytilus galloproviancialis
    Yu, Hong
    Li, Qi
    MOLECULAR ECOLOGY NOTES, 2007, 7 (06): : 1308 - 1310
12345