Development and characterization of 38 novel EST-SSRs for the sea cucumber Apostichopus japonicus

被引：0
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作者：
Wei Peng
Zhenmin Bao
Huixia Du
Ying Dong
Yameng Lv
Zunchun Zhou
Jingjie Hu
机构：
[1] Ocean University of China,Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences
[2] Liaoning Ocean and Fisheries Science Research Institute,Liaoning Key Lab of Marine Fishery Molecular Biology
来源：
Conservation Genetics Resources | 2009年 / 1卷
关键词：
EST; Microsatellite; Sea cucumber;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A total of 5728 sequences from the expressed sequence tag database of sea cucumber Apostichopus japonicus were mined for microsatellite sequences. A total of 151 ESTs containing microsatellites were identified. Of 93 primer pairs designed, 56 amplified expected products and 38 showed polymorphism in 48 individuals tested. The number of alleles ranged from 2 to 10, and the observed and expected heterozygosities varied from 0.000 to 0.900 and from 0.332 to 0.884, respectively. No significant linkage disequilibrium (LD) between pairs of loci was found and 21 loci significantly deviated from the Hardy–Weinberg equilibrium (HWE).
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