Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba

被引：0

作者：

Mederos D.C. ^{[1
,2
,3
]}

Giolitti F. ^{[1
]}

Nome C. ^{[1
]}

Bejerman N. ^{[1
,2
]}

Portal O. ^{[4
]}

机构：

[1] Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Av. 11 de Septiembre 4755, Córdoba

[2] Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, C1425FBQ CABA, Buenos Aires

[3] Departamento de Agronomía, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km 5.5, Santa Clara

[4] Departamento de Biología, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km 5.5, Santa Clara

来源：

Australasian Plant Disease Notes | / 12卷 / 1期

关键词：

Papaya ringspot virus; Sequencing; Sponge gourd;

D O I：

10.1007/s13314-017-0229-6

中图分类号：

学科分类号：

摘要：

Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba. © 2017, Australasian Plant Pathology Society Inc.

引用

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