Annotating genomes with massive-scale RNA sequencing

被引：0

作者：

France Denoeud

Jean-Marc Aury

Corinne Da Silva

Benjamin Noel

Odile Rogier

Massimo Delledonne

Michele Morgante

Giorgio Valle

Patrick Wincker

Claude Scarpelli

Olivier Jaillon

François Artiguenave

机构：

[1] CEA,

[2] DSV,undefined

[3] Institut de Génomique,undefined

[4] CNRS,undefined

[5] UMR 8030,undefined

[6] Université d'Evry,undefined

[7] Scientific and Technology Department,undefined

[8] Istituto di Genomica Applicata,undefined

[9] Parco Scientifico e Tecnologico di Udine,undefined

[10] CRIBI,undefined

[11] Università degli Studi di Padova,undefined

来源：

Genome Biology | / 9卷

关键词：

Alternative Splice; Additional Data File; Coverage Depth; Alternative Splice Event; Massively Parallel Signature Sequencing;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.

引用

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