Micropropagation of Acacia leucophloea (Roxb.) Willd.: A Multi-Purpose Legume Tree

An in vitro micropropagation system has been developed for Acacia leucophloea (Roxb.), an important legume tree of Aravallis in Rajasthan (India). Cotyledonary node segments proved to be the most suitable explants to induce axillary bud proliferation on Murashige and Skoog’s medium containing growth regulators. Nodal segments obtained from 20 to 25 days old in vitro raised seedlings produced a maximum of 4.5 shoots/explant during initiation when medium were supplemented with 1.0 mg l−1 6-benzylaminopurine. For shoot proliferation combination of 2.0 mg l−1 kinetin, 0.5 mg l−1 BAP and 0.05 mg l−1 indole-3-acetic acid produced maximum of 14 shoots/explant. On subsequent culture of shoots on the same fresh medium along with mother explant, an average of 14 shoots/node could be obtained. Rooting could be induced in more than 80% shoots inoculated on MS media containing 1.0 mg l−1 indole-3-butyric acid. The protocol proved to be highly reproducible. © 2017, The National Academy of Sciences, India.