Synchronous luminescence: a simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein

被引：0
|
作者：
Minoo Askari
Gordon Miller
Tuan Vo-Dinh
机构：
[1] Oak Ridge National Laboratory,Advanced Monitoring Development Group, Life Sciences Division
来源：
Biotechnology Letters | 2001年 / 23卷
关键词：
fragile histidine triad gene; fluorescence spectroscopy; hydrolysis; synchronous luminescence spectroscopy;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Human fragile histidine triad (FHIT) protein has dinucleoside 5′,5′′′-P1,Pn-polyphosphates hydrolysis activity, with AMP being one of the reaction products. Application of synchronous luminescence (SL) spectroscopy, in which both excitation and emission wavelengths are scanned simultaneously while a constant wavelength interval is maintained between them, was investigated for detection of the enzymatic activity of the FHIT protein. Ability of SL to identify reaction components, AMP production and its increase as a result of increase in substrate concentration and inhibition of the hydrolysis activity by ZnCl2 are demonstrated.
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页码：1697 / 1702
页数：5
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