Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis

被引：0

作者：

V. G. Sunnichan

K. R. Shivanna

H. Y. Mohan Ram

机构：

[1] Department of Botany,

[2] University of Delhi,undefined

[3] Delhi 110 007,undefined

[4] India,undefined

[5] Department of Environmental Biology,undefined

[6] University of Delhi South Campus,undefined

[7] Benito Juarez Road,undefined

[8] New Delhi 110 021,undefined

[9] India,undefined

来源：

Plant Cell Reports | 1998年 / 17卷

关键词：

Key words Gum karaya; Somatic embryogenesis; Sterculia urens; Thidiazuron; Tree tissue culture;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Nodal explants from selected trees of gum karaya (Sterculia urens Roxb.) in the adult growth phase cultured on Murashige and Skoog (MS) medium supplemented with 6.62 μm N6-benzylaminopurine (BAP) produced an average of six adventitious shoots in 30 days. Shoots were rooted in vitro on 1/4-strength MS medium containing 9.82 μm indole-3-butyric acid. Nodulated callus was produced from hypocotyl explants cultured on MS medium supplemented with 4.52 μm 2,4-dichlorophenoxyacetic acid and 8.90 μm BAP. Somatic embryos developed when the nodulated callus was transferred to MS medium containing 0.45 μm thidiazuron (TDZ). TDZ treatment for 2 days gave the optimum response. Over 30% of the somatic embryos developed into plantlets when transferred to 1/4-strength MS basal medium without any growth regulators. Plantlets produced from adventitious shoots and somatic embryos were acclimatized to ex vitro conditions and established in the field.

引用

页码：951 / 956

页数：5

共 47 条

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