Spectrophotometric Determination of Hydrogen Peroxide Using β-CD-Hemin as a Mimetic Enzyme of Peroxidase

被引：0
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作者：
Yingping Huang
Ruxiu Cai
Luyuan Mao
Zhihong Liu
Houping Huang
机构：
[1] Wuhan university,Department of chemistry
来源：
Analytical Sciences | 1999年 / 15卷
关键词：
Mimetic enzyme; hydrogen peroxide determination; -diethylaniline; spectrophotometry;
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学科分类号：
摘要：
Various mimetic enzymes have been systematically studied concerning the color reaction for hydrogen peroxide oxidizing 4-aminoantipyrine (4-AAP) and N,N-diethylaniline (DEA) to yield a dye. The results have shown that β-CD-hemin is the best mimetic enzyme for peroxidase among those tested, and that DEA is a quite good substrate for β-CD-hemin. Meanwhile, the chromogenic reaction rate and sensitivity of 4-AAP-DEA-β-CD-hemin system for determination of hydrogen peroxide were investigated. Zero to 8.4×10–5 M hydrogen peroxide was determined with good accuracy and reproducibility. The molar-absorption coefficient of the chromogenic compound for hydrogen peroxide determined was found to be 1.65×104 mol l−1 cm−1.
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页码：889 / 894
页数：5
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