A comparative study on physicochemical properties of hydroxyapatite powders derived from natural and synthetic sources

Hydroxy apatite (HA) is effectively used as a bioimplant material because it closely resembles bone apatite and exhibits good biocompatibility. So, in this research, HA powders were produced by calcinations of natural bones including human, bovine, camel and horse bones, and also via sol-gel method. Powders characterizations of natural HA and Synthetic HA were studied by X-ray powder diffraction (XRD), X-ray fluorescence (XRF), Fourier transform infrared (FTIR) and scanning electron microscopy (SEM), combined with transmission electron microscopy (ТЕМ). In order to verify the biocompatibility of these HA powders, MTT assay was applied. The XRD results showed that the HA powders were successfully produced by using different sources. Also, it was obvious from XRF analysis that the main components of them were Ca and P. Furthermore, it was seen that the size of particles was in the nanometric scale and they showed agglomerates consisting of numerous nanocrystals. FTIR spectra of all samples proved the presence of various CO32-, PO43- and OH– groups in the powders. In addition, the MTT assay revealed that the cells proliferations in the presence of horse and human HA nanopowders were stimulated.