Imaging molecular events in single living cells

被引：0
|
作者：
Moritoshi Sato
机构：
[1] The University of Tokyo,Department of Chemistry, School of Science
[2] PRESTO,undefined
[3] Japan Science and Technology Agency,undefined
来源：
Analytical and Bioanalytical Chemistry | 2006年 / 386卷
关键词：
Fluorescent indicator; Fluorescence resonance energy transfer; Second messenger; Protein phosphorylation; Nuclear receptor ligand;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Fluorescence imaging could be the most powerful technique available for observing spatial and temporal dynamics of biomolecules in living cells, if fluorescent indicators for the relevant biomolecules become available. We have recently developed fluorescent indicators for a variety of second messengers or protein phosphorylations. Using the indicators, we have visualized spatial and temporal dynamics of these molecular events in single living cells. These fluorescent indicators are becoming an indispensable tool for understanding the complex mechanism of signal transduction in living cells.
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页码：435 / 443
页数：8
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