Quercetin-loaded chitosan nanoparticles ameliorate adjuvant-induced arthritis in rats by regulating anti-oxidant enzymes and downregulating pro- and inflammatory cytokines

被引:0
|
作者
Abdul Hannan
Bushra Akhtar
Ali Sharif
Fozia Anjum
Imran Pasha
Ahrar Khan
Muhammad Furqan Akhtar
Ammara Saleem
机构
[1] University of Agriculture,Institute of Physiology and Pharmacology
[2] University of Agriculture,Department of Pharmacy
[3] Lahore College for Women University,Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences
[4] Government College University,Department of Chemistry
[5] National Institute of Food Science and Technology,Department of Pharmacology, Faculty of Pharmaceutical Sciences
[6] University of Agriculture,undefined
[7] Shandong Vocational Animal Science and Veterinary College,undefined
[8] Riphah Institute of Pharmaceutical Sciences,undefined
[9] Riphah International University Lahore Campus,undefined
[10] Government College University,undefined
来源
Inflammopharmacology | 2023年 / 31卷
关键词
Quercetin; Rheumatoid arthritis; Nanoparticles; Chitosan;
D O I
暂无
中图分类号
学科分类号
摘要
Rheumatoid arthritis (RA) is an inflammatory condition and associated with the symmetrical synovitis of the joints and cause joint pain. The use of anti-rheumatic drugs is associated with many adverse effects. Quercetin, an important polyphenolic flavonoid, possess anti-inflammatory and anti-rheumatic effects. Quercetin use is limited due to poor absorption and bioavailability. Nanomedicines are used for the targeted drug delivery, hence it reduces the adverse effects of the drug. Based upon these factors, quercetin-loaded chitosan nanoparticles (Q-NPs) were prepared by solvent evaporation method, characterized and their better anti-rheumatic effect with mechanistic insights was validated in Freund’s complete adjuvant (FCA)-induced arthritic rats along with safety studies. The animals were divided into five groups, each containing 5 animals. Group I was normal control, group II was arthritic control, while groups III, IV and V were administered with quercetin (15 mg/Kg) and Q-NPs (10 and 20 mg/Kg), respectively. The reduction in ankle diameter, serum oxidative stress markers as well as pro- and inflammatory cytokines, e.g., tumor necrosis factor (TNFα), interleukin (IL-6) were determined. The prepared Q-NPs showed hydrodynamic size of 83.9 nm, polydispersity index of 0.687, entrapment efficiency 90.5% as well as no interaction between quercetin and chitosan in Fourier transform infrared spectroscopy (FTIR). A significant reduction (p < 0.001) in ankle diameter was observed after administration of high-dose Q-NPs (4.32 ± 0.14 cm to 5.13 ± 0.62 cm). There was also reduction (p < 0.001) in levels of TNFα and IL-6 following high-dose Q-NPs (72.56 ± 2.30 and 308.19 ± 11.5 pg). The effect on biochemical tests, hematological parameters and oxidative stress parameters was also found to be significant. Histopathological changes of kidney, liver and ankle also confirmed the anti-rheumatic effect of high-dose Q-NPs. The study concludes that administration of Q-NPs (20 mg/Kg) may be used for the treatment of FCA-induced RA in rats.
引用
收藏
页码:287 / 300
页数:13
相关论文
共 21 条
  • [1] Quercetin-loaded chitosan nanoparticles ameliorate adjuvant-induced arthritis in rats by regulating anti-oxidant enzymes and downregulating pro- and inflammatory cytokines
    Hannan, Abdul
    Akhtar, Bushra
    Sharif, Ali
    Anjum, Fozia
    Pasha, Imran
    Khan, Ahrar
    Akhtar, Muhammad Furqan
    Saleem, Ammara
    [J]. INFLAMMOPHARMACOLOGY, 2023, 31 (01) : 287 - 300
  • [2] Curcumin loaded solid lipid nanoparticles ameliorate adjuvant-induced arthritis in rats
    Arora, R.
    Kuhad, A.
    Kaur, I. P.
    Chopra, K.
    [J]. EUROPEAN JOURNAL OF PAIN, 2015, 19 (07) : 940 - 952
  • [3] Methotrexate-loaded biodegradable nanoparticles exert anti-arthritic effect by downregulating pro-inflammatory cytokines in Freund’s complete adjuvant-induced arthritic rats
    Muhammad Usman Saleem
    Faqir Muhammad
    Ali Sharif
    Muhammad Imran Arshad
    Kanwal Akhtar
    Yasir Javed
    Bushra Akhtar
    [J]. Inflammopharmacology, 2022, 30 : 1079 - 1091
  • [4] Methotrexate-loaded biodegradable nanoparticles exert anti-arthritic effect by downregulating pro-inflammatory cytokines in Freund's complete adjuvant-induced arthritic rats
    Saleem, Muhammad Usman
    Muhammad, Faqir
    Sharif, Ali
    Arshad, Muhammad Imran
    Akhtar, Kanwal
    Javed, Yasir
    Akhtar, Bushra
    [J]. INFLAMMOPHARMACOLOGY, 2022, 30 (03) : 1079 - 1091
  • [5] Synthesized pyrrole ester ameliorates adjuvant-induced arthritis in Wistar rats by alleviating inflammation and downregulating the pro-inflammatory cytokines
    Kimariyo, Patrick Francis
    Kurati, Sony Priya
    Bhargavi, Samireddi Naga Venkata Durga
    Gordon, Andrew
    Kayabu, Dickson
    Muthyala, Murali Krishna Kumar
    [J]. INFLAMMOPHARMACOLOGY, 2024, 32 (04) : 2361 - 2375
  • [6] Ellagic acid alleviates adjuvant induced arthritis by modulation of pro- and anti-inflammatory cytokines
    Allam, Gamal
    Mahdi, Emad A.
    Alzahrani, Abdullah M.
    Abuelsaad, Abdelaziz S.
    [J]. CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY, 2016, 41 (04) : 339 - 349
  • [7] Anti-inflammatory effects and hepatotoxicity of Tripterygium-loaded solid lipid nanoparticles on adjuvant-induced arthritis in rats
    Xue, Mei
    Jiang, Zhen-zhou
    Wu, Tao
    Li, Ji
    Zhang, Liang
    Zhao, Yan
    Li, Xue-jun
    Zhang, Lu-Yong
    Yang, Shu-yu
    [J]. PHYTOMEDICINE, 2012, 19 (11) : 998 - 1006
  • [8] Investigation of the Anti-inflammatory and Anti-oxidant Effects of Phosphodiesterase-5 Inhibitor Tadalafil on Rat Adjuvant-induced Model of Arthritis
    Bahadir, Elif F.
    Koroglu, Kutay M.
    Yuksel, Meral
    Ercan, Feriha
    Alican, Inci
    [J]. ACTA PHYSIOLOGICA, 2016, 218 : 18 - +
  • [9] Anti-inflammatory effects of triptolide loaded poly(D,L-lactic acid) nanoparticles on adjuvant-induced arthritis in rats
    Liu, MX
    Dong, J
    Yang, YJ
    Yang, XL
    Xu, HB
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2005, 97 (02) : 219 - 225
  • [10] Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (Turmeric) Versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant-Induced Arthritis
    Gamal Ramadan
    Mohammed Ali Al-Kahtani
    Wael Mohamed El-Sayed
    [J]. Inflammation, 2011, 34 : 291 - 301