Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter

被引：0

作者：

A. M. M. Attia

W. M. Aboulthana

G. M. Hassan

E. Aboelezz

机构：

[1] National Research Centre,Genetic Engineering and Biotechnology Division, Biochemistry Department

[2] National Institute of Standards,Division of Thermometry and Ionizing Radiation Metrology, Department of Ionizing Radiation Metrology

来源：

Radiation and Environmental Biophysics | 2020年 / 59卷

关键词：

Biological dosimetry; γ-Irradiation; Carboxyhemoglobin (HbCO); Methemoglobin (MetHb); Oxyhemoglobin (HbO; ); Sulfhemoglobin (SHb);

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Biological dosimetry based on sulfhemoglobin (SHb), methemoglobin (MetHb), and carboxyhemoglobin (HbCO) levels was evaluated. SHb, MetHb and HbCO levels were estimated in erythrocytes of mice irradiated by γ rays from a 60Co source using the method of multi-component spectrophotometric analysis developed recently. In this method, absorption measurements of diluted aqueous Hb-solution were made at λ = 500, 569, 577 and 620 nm, and using the mathematical formulas based on multi-component spectrophotometric analysis and the mathematical Gaussian elimination method for matrix calculation, the concentrations of various Hb-derivatives and total Hb in mice blood were estimated. The dose range of γ rays was from 0.5 to 8 Gy and the dose rate was 0.5 Gy min−1. Among all Hb-derivatives, MetHb, SHb and HbCO demonstrated an unambiguous dose-dependent response. For SHb and MetHb, the detection limits were about 0.5 Gy and 1 Gy, respectively. After irradiation, high levels of MetHb, SHb and HbCO persisted for at least 10 days, and the maximal increase of MetHb, SHb and HbCO occurred up to 24 h following γ irradiation. The use of this “MetHb + SHb + HbCO”-derivatives-based absorbed dose relationship showed a high accuracy. It is concluded that simultaneous determination of MetHb, SHb and HbCO, by multi-component spectrophotometry provides a quick, simple, sensitive, accurate, stable and inexpensive biological indicator for the early assessment of the absorbed dose in mice.

引用

页码：131 / 144

页数：13

共 23 条

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[22] Gamma and infrared novel methodologies in Aerospace re-entry: γ-rays crystal efficiency by GEANT4 for TPS material recession assessment and simultaneous dual color infrared temperature determination
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[23] Gamma and infrared novel methodologies in aerospace re-entry: γ-rays crystal efficiency by GEANT4 for TPS material recession assessment and simultaneous dual color infrared temperature determination (vol 479, pg 264, 2020)
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Savino, L.
Di Leva, A.
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