ProFiT: An MS-based method for skeletal muscle fiber typing

被引：0
|
作者：
Kallabis S. [1 ]
Krüger M. [1 ,2 ]
机构：
[1] CECAD Forschungszentrum, Universität zu Köln, Joseph-Stelzmann-Straße 26, Köln
[2] Zentrum für Molekulare Medizin Köln (ZMMK), Universität ZU Köln, Köln
来源：
BIOspektrum | 2021年 / 27卷 / 7期
关键词：
D O I：
10.1007/s12268-021-1671-8
中图分类号：
学科分类号：
摘要：
Skeletal muscle tissue is composed of different fiber types which differ in their metabolic activity and molecular expression patterns. Mass spectrometric analyses revealed muscle fiber-specific traits but the number of detected proteins is limited due to low protein abundance and high dynamic range. We have developed a new LC-MS/MS method to circumvent the bottleneck of low protein abundance and we have applied this method to measure fiber type-specific phosphoproteomes for the first time. © 2021, Die Autoren.
引用
收藏
页码：719 / 722
页数：3
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