Isolation and characterization of 33 EST-SNP markers in Schizothorax prenanti

Prenant’s schizothoracin, Schizothorax prenanti (Tchang), is one of the most important cold-water economic fish species in China. In this study, we reported the isolation and characterization of 33 SNP markers among 30 individuals from one cultured population. The observed and expected heterozygosities ranged from 0.0312 to 0.8438 and 0.0312 to 0.5079, respectively. Polymorphic information content ranged from 0.0302 to 0.3747. Significant deviation from HWE was observed for 5 loci and no significant linkage disequilibrium was found between pairs of loci. These EST-SNP markers are potentially useful for the study of population genetics, construction of genetic linkage and QTL maps in S. prenanti.