A New Method for Purification of Carbonic Anhydrase Isozymes by Affinity Chromatography

被引：0
|
作者：
O. Ozensoy
O. Arslan
S. Oznur Sinan
机构：
[1] Balikesir University,Science and Art Faculty, Department of Chemistry/Biochemistry Section
[2] Balikesir University,Science and Art Faculty, Department of Biology/Biochemistry Section
来源：
Biochemistry (Moscow) | 2004年 / 69卷
关键词：
EUPERGIT; C-250L; carbonic anhydrase; affinity chromatography;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A new affinity gel for purification of carbonic anhydrase isozymes was prepared using EUPERGITR C-250L derivatized with p-aminobenzenesulfonamide, an inhibitor of carbonic anhydrase. The binding capacity of the affinity gel was determined at different temperatures, pH values, elution buffers, and ionic strengths. Human carbonic anhydrase isozymes (HCA I and HCA II) and bovine carbonic anhydrase (BCA) were purified in high yields from erythrocytes.
引用
收藏
页码：216 / 219
页数：3
相关论文
共 50 条
  • [21] CARBONIC-ANHYDRASE AND CARBOXYPEPTIDASE-A METALLOPROTEIN AFFINITY METAL CHROMATOGRAPHY
    LITAKER, J
    DONALD, S
    SPEARS, R
    VINCENT, JB
    MINERALS AND METALLURGICAL PROCESSING, 1995, 12 (02): : 108 - 111
  • [22] Structure, function and applications of carbonic anhydrase isozymes
    Hassan, Md Imtaiyaz
    Shajee, Bushra
    Waheed, Abdul
    Ahmad, Faizan
    Sly, William S.
    BIOORGANIC & MEDICINAL CHEMISTRY, 2013, 21 (06) : 1570 - 1582
  • [23] ZINC ENVIRONMENTAL DIFFERENCES IN CARBONIC ANHYDRASE ISOZYMES
    WARD, RL
    BIOCHEMISTRY, 1970, 9 (12) : 2447 - +
  • [24] A new affinity gel for the purification of α-carbonic anhdrases
    Sahin, Aysegul
    Isik, Semra
    Arslan, Oktay
    Supuran, Claudiu T.
    Guler, Ozen Ozensoy
    JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 2015, 30 (02) : 224 - 228
  • [25] Purification method for human plasma kallikrein by a new affinity chromatography
    Tada, M
    Wanaka, K
    Okamoto, S
    Okamoto, U
    Nakaya, Y
    Horie, N
    Hijikata-Okunomiya, A
    Tsuda, Y
    Okada, Y
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1998, 21 (02) : 105 - 108
  • [26] PURIFICATION OF GLYCOGEN-PHOSPHORYLASE ISOZYMES BY METAL-AFFINITY CHROMATOGRAPHY
    LUONG, CBH
    BROWNER, MF
    FLETTERICK, RJ
    HAYMORE, BL
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 584 (01): : 77 - 84
  • [27] A METHOD FOR PURIFICATION OF ACETYLCHOLINESTERASE BY AFFINITY CHROMATOGRAPHY
    KALDERON, N
    SILMAN, I
    BLUMBERG, S
    DUDAI, Y
    BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 207 (03) : 560 - &
  • [28] Chromatographic and electrophoretic methods related to the carbonic anhydrase isozymes
    Bergenhem, N
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1996, 684 (1-2): : 289 - 305
  • [29] IMMUNOHISTOCHEMICAL LOCALIZATION OF HUMAN CARBONIC-ANHYDRASE ISOZYMES
    KUMPULAINEN, T
    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1984, 429 (JUN) : 359 - 368
  • [30] Carbonic anhydrase isozymes IX and XII in gastric tumors
    Leppilampi, M
    Saarnio, J
    Karttunen, TJ
    Kivelä, J
    Pastoreková, S
    Pastorek, J
    Waheed, A
    Sly, WS
    Parkkila, S
    WORLD JOURNAL OF GASTROENTEROLOGY, 2003, 9 (07) : 1398 - 1403
12345