Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from hodococcus erythropolis

被引：0

作者：

Ch. Reisinger

I. Osprian

A. Glieder

H. E. Schoemaker

H. Griengl

H. Schwab

机构：

[1] Graz University of Technology,Institute of Molecular Biotechnology

[2] Graz University of Technology,Institute of Organic Chemistry

[3] Competence Centre Applied Biocatalysis,Van ’t Hoff Institute for Molecular Sciences

[4] University of Amsterdam,undefined

来源：

Biotechnology Letters | 2004年 / 26卷

关键词：

amidase; biotransformation; cyanohydrin hydrolysis; nitrile hydratase;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli.Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding Î±-hydroxy amides and acids while retaining enantiopurity.

引用

页码：1675 / 1680

页数：5

共 50 条

[41] Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564:: preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes
Kashiwagi, M
Fuhshuku, KI
Sugai, T
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2004, 29 (1-6) : 249 - 258
[42] STUDY OF PENICILLIN AMIDASE FROM ESCHERICHIA-COLI - PH-DEPENDENCE OF EQUILIBRIUM-CONSTANT FOR ENZYMATIC-HYDROLYSIS OF AMPICILLIN
MARGOLIN, AL
SHVYADAS, VYK
NYS, PS
KOLTSOVA, EV
SAVITSKAYA, EM
BEREZIN, IV
ANTIBIOTIKI, 1978, 23 (02): : 114 - 118
[43] AMIDASE COUPLED WITH LOW-MOLECULAR-MASS NITRILE HYDRATASE FROM RHODOCOCCUS-RHODOCHROUS J1 - SEQUENCING AND EXPRESSION OF THE GENE AND PURIFICATION AND CHARACTERIZATION OF THE GENE-PRODUCT
KOBAYASHI, M
KOMEDA, H
NAGASAWA, T
NISHIYAMA, M
HORINOUCHI, S
BEPPU, T
YAMADA, H
SHIMIZU, S
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 217 (01): : 327 - 336
[44] STUDY OF PENICILLIN AMIDASE FROM ESCHERICHIA-COLI PH-DEPENDENCE OF EQUILIBRIUM-CONSTANT FOR ENZYMATIC-HYDROLYSIS OF BENZYLPENICILLIN
BEREZIN, IV
KLESOV, AA
MARGOLIN, AL
NYS, PS
SAVITSKAYA, EM
SHVYADAS, VK
ANTIBIOTIKI, 1976, 21 (06): : 519 - 523
[45] PRODUCTION OF S-(+)-2-PHENYLPROPIONIC ACID FROM (R,S)-2-PHENYLPROPIONITRILE BY THE COMBINATION OF NITRILE HYDRATASE AND STEREOSELECTIVE AMIDASE IN RHODOCOCCUS-EQUI TG328
GILLIGAN, T
YAMADA, H
NAGASAWA, T
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1993, 39 (06) : 720 - 725
[46] Synergistically Enhanced Enzymatic Hydrolysis of Sugarcane Bagasse Mediated by a Recombinant Endo-Xylanase from Streptomyces ipomoeae
Li, Zhong
Dong, Youqing
Liu, Junli
Xian, Liang
Tang, Aixing
Li, Qingyun
Li, Qunliang
Liu, Youyan
PROCESSES, 2024, 12 (09)
[47] Enzymatic nitrile hydrolysis catalyzed by nitrilase ZmNIT2 from maize.: An unprecedented β-hydroxy functionality enhanced amide formation
Mukherjee, Chandrani
Zhu, Dunming
Biehl, Edward R.
Parmar, Rajiv R.
Hua, Ling
TETRAHEDRON, 2006, 62 (26) : 6150 - 6154
[48] Efficient Enzymatic Hydrolysis and Polyhydroxybutyrate Production from Non-Recyclable Fiber Rejects from Paper Mills by Recombinant Escherichia coli
Jia, Linjing
Juneja, Ankita
Majumder, Erica L. -W.
Ramarao, Bandaru V.
Kumar, Deepak
PROCESSES, 2024, 12 (08)
[49] Transcriptional analysis of the nitrile-degrading operon from Rhodococcus sp ACV2 and high level production of recombinant amidase with an Escherichia coli-T7 expression system
Bigey, F
Chebrou, H
Fournand, D
Arnaud, A
JOURNAL OF APPLIED MICROBIOLOGY, 1999, 86 (05) : 752 - 760
[50] Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli
Dexi Wu
Qiang Li
Dan Wang
Yugang Dong
Applied Biochemistry and Biotechnology, 2013, 170 : 1942 - 1949

← 1 2 3 4 5 →