Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from hodococcus erythropolis

被引：0
|
作者：
Ch. Reisinger
I. Osprian
A. Glieder
H. E. Schoemaker
H. Griengl
H. Schwab
机构：
[1] Graz University of Technology,Institute of Molecular Biotechnology
[2] Graz University of Technology,Institute of Organic Chemistry
[3] Competence Centre Applied Biocatalysis,Van ’t Hoff Institute for Molecular Sciences
[4] University of Amsterdam,undefined
来源：
Biotechnology Letters | 2004年 / 26卷
关键词：
amidase; biotransformation; cyanohydrin hydrolysis; nitrile hydratase;
D O I：
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中图分类号：
学科分类号：
摘要：
Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli.Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding α-hydroxy amides and acids while retaining enantiopurity.
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页码：1675 / 1680
页数：5
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