Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates

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作者
Takuya Hasegawa
Atsushi Okabe
Yusuke Kato
Atsushi Ooshiro
Hiroshi Kawaide
Masahiro Natsume
机构
[1] Tokyo University of Agriculture and Technology,Department of Biological Production Science, United Graduate School of Agricultural Science
[2] Tokyo University of Agriculture and Technology,Department of Bioregulation and Biointeraction, Graduate School of Agriculture
[3] Okinawa Prefectural Agricultural Research Center,Division of Bioregulation and Biointeraction, Institute of Agriculture
[4] Tokyo University of Agriculture and Technology,undefined
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关键词
Bacterial wilt; Chemotaxis; Tomato; Root exudates;
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摘要
Tomato root exudates were analyzed using a bioassay to detect the chemoattractant for Ralstonia solanacearum. An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity for R. solanacearum strain MAFF 730138. The active component, purified using a Sep-Pak C18 cartridge, an activated charcoal column, diol-modified silica gel, and NH2-modified silica gel, is a new hydrophobic attractant. The final purified fraction produced a single peak in a diol-modified silica gel HPLC analysis.
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页码:20 / 26
页数:6
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