Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion

被引：0
|
作者：
Xiaoxia Liu
Alpay Burak Seven
Junjie Xu
Victoria Esser
Lijing Su
Cong Ma
Josep Rizo
机构：
[1] University of Texas Southwestern Medical Center,Department of Biophysics
[2] University of Texas Southwestern Medical Center,Department of Biochemistry
[3] University of Texas Southwestern Medical Center,Department of Pharmacology
[4] Key Laboratory of Molecular Biophysics of the Ministry of Education,undefined
[5] College of Life Science and Technology,undefined
[6] Huazhong University of Science and Technology,undefined
来源：
Nature Protocols | 2017年 / 12卷
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摘要：
This protocol monitors how fusion of proteoliposomes containing the vesicle-associated SNARE synaptobrevin with proteoliposomes containing the target-associated SNAREs syntaxin-1 and SNAP-25 depends on Munc18-1, Munc13-1, synaptotagmin-1, NSF and α-SNAP.
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页码：2014 / 2028
页数：14
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