HPLC–ICP–MS determination of selenium distribution and speciation in different types of nut

被引:0
|
作者
Sasi S. Kannamkumarath
Kazimierz Wrobel
Katarzyna Wrobel
Anne Vonderheide
Joseph A. Caruso
机构
[1] Department of Chemistry,
[2] University of Cincinnati,undefined
[3] Cincinnati,undefined
[4] Ohio,undefined
[5] 45221–0172,undefined
来源
Analytical and Bioanalytical Chemistry | 2002年 / 373卷
关键词
HPLC–ICP–MS Nuts Proteinase K Selenomethionine Speciation;
D O I
暂无
中图分类号
学科分类号
摘要
In addition to determination of total selenium in nuts, the element distribution among different fractions (lipid extract, low molecular weight, and protein fractions), and speciation analysis were studied. Improved precision for total selenium determination was observed after elimination of lipids. Because selenium was not detected in any of the lipid extracts obtained from the different types of nuts (ICP–MS), in each determination and/or speciation procedure used in this work lipids were extracted (chloroform–methanol, 2:1) and discarded before analysis. In agreement with previously reported data, high selenium levels were found in Brazil nuts (those purchased without shells contained approximately a quarter the content than those purchased with shells) and significantly lower levels in walnuts, cashews, and pecans nuts. Low-molecular-weight compounds were extracted with perchloric acid (0.4 mol L–1) to furnish a fraction containing 3 to 15% of the total selenium in different types of nuts. The proteins were isolated from nut samples by dissolution in 0.1 mol L–1 sodium hydroxide and subsequent precipitation with acetone. They were then dissolved in phosphate buffer pH 7.5. Analysis of protein fractions focused on selenium in two possible states – weakly and firmly bound to proteins. Results obtained for Brazil nuts by size-exclusion chromatography with on-line ICP–MS detection, in the absence and in the presence of β-mercaptoethanol, showed that approximately 12% of total selenium was weakly bound to proteins. To obtain information about firmly bound selenium, the protein extracts were hydrolyzed enzymatically with proteinase K. Speciation was performed by means of ion-pairing HPLC–ICP–MS. The primary species found in all types of nuts was Se-methionine (19–25% of total selenium for different types of nuts).
引用
收藏
页码:454 / 460
页数:6
相关论文
共 50 条
  • [31] Chiral speciation of selenoamino acids and selenium enriched samples using HPLC coupled to ICP-MS
    Ponce de Leon, Claudia A., 1600, Royal Soc of Chemistry, Cambridge, United Kingdom (15):
  • [32] Inorganic selenium speciation using HPLC-ICP-hexapole collision/reaction cell-MS
    Mazan, S
    Gilon, N
    Crétier, G
    Rocca, JL
    Mermet, JM
    JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2002, 17 (04) : 366 - 370
  • [33] Chiral speciation of selenoamino acids and selenium enriched samples using HPLC coupled to ICP-MS
    de Leon, CAP
    Sutton, KL
    Caruso, JA
    Uden, PC
    JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2000, 15 (09) : 1103 - 1107
  • [34] Selenium speciation studies in Se-enriched chives (Allium schoenoprasum) by HPLC-ICP-MS
    Kapolna, Emese
    Shah, Monika
    Caruso, Joseph A.
    Fodor, Peter
    FOOD CHEMISTRY, 2007, 101 (04) : 1398 - 1406
  • [35] An attempt to differentiate HPLC-ICP-MS selenium speciation in natural and selenised Agaricus mushrooms using different species extraction procedures
    Huerta, VD
    Sánchez, MLF
    Sanz-Medel, A
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 384 (04) : 902 - 907
  • [36] An attempt to differentiate HPLC-ICP-MS selenium speciation in natural and selenised Agaricus mushrooms using different species extraction procedures
    Vanesa Díaz Huerta
    María Luisa Fernández Sánchez
    Alfredo Sanz-Medel
    Analytical and Bioanalytical Chemistry, 2006, 384 : 902 - 907
  • [37] Studying the distribution pattern of selenium in nut proteins with information obtained from SEC-UV-ICP-MS and CE-ICP-MS
    Kannamkumarath, SS
    Wrobel, K
    Wuilloud, RG
    TALANTA, 2005, 66 (01) : 153 - 159
  • [38] Determination of selenium species in biota with an emphasis on animal tissues by HPLC-ICP-MS
    Jagtap, Rajani
    Maher, William
    MICROCHEMICAL JOURNAL, 2016, 124 : 422 - 529
  • [39] Determination of selenium species in black bean protein powder by HPLC and ICP-MS
    Meng, L.
    Xu, Y. L.
    He, B.
    Qi, M.
    Xia, Z. R.
    Tang, D. J.
    SELENIUM RESEARCH FOR ENVIRONMENT AND HUMAN HEALTH: PERSPECTIVES, TECHNOLOGIES AND ADVANCEMENTS, 2020, : 209 - 210
  • [40] Simultaneous determination of arsenic, selenium and antimony species using HPLC/ICP-MS
    Lindemann, T
    Prange, A
    Dannecker, W
    Neidhart, B
    FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1999, 364 (05): : 462 - 466