Cloning and sequencing of a gene encoding a novel salt stress-induced membrane protein from Rhodobacter sphaeroides f. sp. denitrificans

被引：0
|
作者：
XY. Xu
H. Kadokura
A. Okubo
K. Kitamoto
S. Yamazaki
机构：
[1] Department of Applied Biological Chemistry,
[2] The University of Tokyo,undefined
[3] 1–1-1 Yayoi,undefined
[4] Bunkyo-ku,undefined
[5] Tokyo 113–8657,undefined
[6] Japan,undefined
[7] Department of Biotechnology,undefined
[8] The University of Tokyo,undefined
[9] 1–1-1 Yayoi,undefined
[10] Bunkyo-ku,undefined
[11] Tokyo 113–8657,undefined
[12] Japan,undefined
来源：
Applied Microbiology and Biotechnology | 2001年 / 56卷
关键词：
Peptide; Stress Condition; Polypeptide; Heat Shock; Membrane Protein;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The gene encoding a membrane protein, SspA, induced under salt stress conditions was cloned and sequenced from a photosynthetic bacterium, Rhodobacter sphaeroides f. sp. denitrificans IL106. A single open reading frame consisting of 972 base pairs that encoded a polypeptide composed of a signal peptide of 24 amino acids and a mature protein of 300 amino acids (Mr 33,386) was found. A database search failed to detect any highly homologous sequences, indicating that SspA is a novel protein. The protein was present in the outer membrane as a transmembrane protein and was specifically induced by salt stress, but not by heat shock.
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页码：442 / 447
页数：5
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