Fluorescence lifetime-resolved imaging

被引：0

作者：

Yi-Chun Chen

Robert M. Clegg

机构：

[1] University of Illinois at Urbana-Champaign,Bioengineering Department

[2] University of Illinois at Urbana-Champaign,Center for Biophysics and Computational Biology

[3] University of Illinois at Urbana-Champaign,Department of Physics

[4] Loomis Laboratory of Physics,undefined

来源：

Photosynthesis Research | 2009年 / 102卷

关键词：

Fluorescence lifetime-resolved imaging; Microscopy; Model independent analysis; Fluorescence lifetime-resolved imaging microscopy; Fluorescence lifetime-resolved imaging; Spectral FLIM; Time-domain; Frequency domain;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.

引用

页码：143 / 155

页数：12

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