A modified mini-prep method for economical and rapid extraction of genomic DNA in plants

被引：35
|
作者：
Khan I.A. [1 ]
Awan F.S. [1 ]
Ahmad A. [1 ]
Khan A.A. [1 ]
机构：
[1] Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad
来源：
Plant Molecular Biology Reporter | 2004年 / 22卷 / 1期
关键词：
Cotton; DNA extraction method; Molecular markers; Wheat;
D O I：
10.1007/BF02773355
中图分类号：
学科分类号：
摘要：
Molecular markers for map-based cloning, marker-assisted selection in crop breeding, and genetic studies require DNA isolation from a large number of plants in a short span of time. Here we describe a modified DNA extraction method that is economical in terms of cost, time, and labour. The method allows DNA extraction from as little as 0.2-0.3 g of leaves that are homogenized in zipper plastic bags, followed by DNA isolation in 1.5-mL Eppendorf tubes. By using the modified method, a DNA yield of 700-800 μg/300 mg leaf tissue was obtained from cotton and wheat samples. The quality of the DNA was quite suitable for PCR-based markers. © 2004 International Society for Plant Molecular Biology.
引用
收藏
页码：89a / 89e
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