Standardized RT-PCR Conditions for Detection and Identification of Eleven Viruses of Potato and Potato spindle tuber viroid

被引：0
|
作者：
James M. Crosslin
Launa L. Hamlin
机构：
[1] Agricultural Research Service,USDA
[2] USDA-ARS Vegetable and Forage Crops Research Unit,undefined
来源：
American Journal of Potato Research | 2011年 / 88卷
关键词：
Potato diseases; Plant viruses;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Standardized RT-PCR procedures were developed and validated for detection of Alfalfa mosaic virus (AMV), Impatiens necrotic spot virus (INSV), Tobacco rattle virus (TRV), Tomato spotted wilt virus (TSWV), Potato leaf roll virus (PLRV), Potato mop top virus (PMTV), Potato virus A (PVA), Potato virus M (PVM), Potato virus S (PVS), Potato virus X (PVX), Potato virus Y (PVY), and Potato spindle tuber viroid (PSTVd). Under the same conditions the O, N, NTN, and N:O strains of PVY could be differentiated using previously published multiplex primers.
引用
收藏
页码：333 / 338
页数：5
相关论文
共 50 条
  • [11] Duplex realtime RT-PCR assay for the detection of Potato spindle tuber viroid (PSTVd) along with ef 1-α gene of potato
    Jeevalatha, A.
    Kumar, Ravinder
    Raigond, Baswaraj
    Sundaresha, S.
    Sharma, Sanjeev
    Singh, B. P.
    PHYTOPARASITICA, 2015, 43 (03) : 317 - 325
  • [12] Detection of potato spindle tuber viroid (PSTVd) in minute amounts of potato (Solanum tuberosum L.) leaf tissue by hybridization techniques and, together with potato viruses, by multiplex RT-PCR
    Khan, M. S.
    Timmermann, C.
    Hoque, M. I.
    Sarker, R. H.
    Muehlbach, H.-P.
    JOURNAL OF PLANT DISEASES AND PROTECTION, 2009, 116 (03) : 97 - 105
  • [13] Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber
    Peiman, M.
    Xie, C.
    AUSTRALASIAN PLANT DISEASE NOTES, 2006, 1 (01): : 41 - 46
  • [14] Sensitive detection of potato viruses, PVX, PLRV and PVS, by RT-PCR in potato leaf and tuber
    M. Peiman
    C. Xie
    Australasian Plant Disease Notes, 2006, 1 (1): : 41 - 46
  • [15] DETECTION OF POTATO SPINDLE TUBER VIROID IN TRUE POTATO SEED
    GRASMICK, ME
    SLACK, SA
    PHYTOPATHOLOGY, 1982, 72 (07) : 962 - 962
  • [16] POTATO SPINDLE TUBER VIROID
    SINGH, RP
    CANADIAN PLANT DISEASE SURVEY, 1984, 64 (01): : 2 - 2
  • [17] Potato spindle tuber viroid
    Kochetov, A., V
    Pronozin, A. Y.
    Shatskaya, N., V
    Afonnikov, D. A.
    Afanasenko, O. S.
    VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII, 2021, 25 (03): : 269 - 275
  • [18] “Development and comparison of reverse transcription-loop-mediated isothermal amplification assay (RT-LAMP), RT-PCR and real time PCR for detection of Potato spindle tuber viroid in potato”
    Gaurav Verma
    Baswaraj Raigond
    Shruti Pathania
    Tarvinder Kochhar
    Kailash Naga
    European Journal of Plant Pathology, 2020, 158 : 951 - 964
  • [19] Development and evaluation of a multiplex RT-PCR for detecting main viruses and a viroid of potato
    Peiman, M.
    Xie, C.
    ACTA VIROLOGICA, 2006, 50 (02) : 129 - 133
  • [20] DEVELOPMENT OF A STANDARD METHOD FOR DETECTION OF POTATO SPINDLE TUBER VIROID IN POTATO PLANTS
    MOSCH, WHM
    HUTTINGA, H
    MAAT, DZ
    TREUR, A
    NETHERLANDS JOURNAL OF PLANT PATHOLOGY, 1982, 88 (03): : 113 - 122
12345