Standardized RT-PCR Conditions for Detection and Identification of Eleven Viruses of Potato and Potato spindle tuber viroid

被引:0
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作者
James M. Crosslin
Launa L. Hamlin
机构
[1] Agricultural Research Service,USDA
[2] USDA-ARS Vegetable and Forage Crops Research Unit,undefined
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关键词
Potato diseases; Plant viruses;
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摘要
Standardized RT-PCR procedures were developed and validated for detection of Alfalfa mosaic virus (AMV), Impatiens necrotic spot virus (INSV), Tobacco rattle virus (TRV), Tomato spotted wilt virus (TSWV), Potato leaf roll virus (PLRV), Potato mop top virus (PMTV), Potato virus A (PVA), Potato virus M (PVM), Potato virus S (PVS), Potato virus X (PVX), Potato virus Y (PVY), and Potato spindle tuber viroid (PSTVd). Under the same conditions the O, N, NTN, and N:O strains of PVY could be differentiated using previously published multiplex primers.
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页码:333 / 338
页数:5
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