Protein quantification by the SELDI-TOF-MS–based ProteinChip® System

被引：0
|
作者：
Sonja Vorderwülbecke
Steve Cleverley
Scot R Weinberger
Andreas Wiesner
机构：
[1] Ciphergen Biosystems GmbH,
[2] Hannah-Vogt-Str. 1,undefined
[3] Ciphergen Biosystems Ltd.,undefined
[4] Ciphergen Biosystems Inc.,undefined
来源：
Nature Methods | 2005年 / 2卷 / 5期
关键词：
D O I：
10.1038/nmeth0505-393
中图分类号：
学科分类号：
摘要：
Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) represents the successful combination of retentate chromatography and mass spectrometry, and this technology is an integral part of Ciphergen's ProteinChip System, which was designed to answer biomedical questions by performing protein analyses on a single experimental platform. The quantification capability of the ProteinChip System is essential in all proteomic applications for which this technology is used. Here we describe methods and results for three short experiments mimicking realistic analytical challenges to provide practical examples of quantification.
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页码：393 / 395
页数：2
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