Transformation of triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment

被引：0
|
作者：
G. Zhang
S. Lu
T. A. Chen
C. R. Funk
W. A. Meyer
机构：
[1] Rutgers University,Department of Plant Biology and Pathology
[2] South China Agricultural University,College of Biotechnology
来源：
Plant Cell Reports | 2003年 / 21卷
关键词：
Triploid bermudagrass; Transformation; Biolistics; Hygromycin phosphotransferase gene;
D O I：
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中图分类号：
学科分类号：
摘要：
A transformation system for triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 μM dicamba, 20 μM benzylaminopurine, and 100 mg/l myo-inositol. Using the hygromycin phosphotransferase (hpt) gene as the selectable marker gene, we obtained 75 transgenic lines from 18 petri dishes bombarded. Integration of the hpt gene into genomic DNA and transcription of hpt was confirmed by Southern and Northern blot analyses, respectively. Through suspension culture screening, we obtained homogeneously transformed plants showing stable transcription of the hpt gene.
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页码：860 / 864
页数：4
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